Use of xenon and krypton for phasing in protein crystallography
نویسندگان
چکیده
Xenon derivatisation is classically performed with hydrophilic additives (e.g. glycerol) to prevent nucleation of ice when the cryoprotectant buffer containing the crystal is flash cooled. The difficulties of obtaining a suitable cryoprotectant buffer, in which crystals are stable for a period of several minutes are, however, rarely reported. Crystals of porcine pancreatic elastase have been used to demonstrate that cryoprotection using dry paraffin oil [1] or Panjelly allows with equal or better efficacy derivatisation under a xenon atmosphere prior to shock cooling [2].
منابع مشابه
MAD phasing with krypton.
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